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    一株煙草花葉病毒生防細(xì)菌的鑒定及代謝物活性研究

    Identification and Antivirus Activity of Bacteria Strain By33 Against Tobacco Mosaic Virus

    • 摘要: 從煙田土樣中分離到1 株對TMV 有拮抗作用的細(xì)菌By33，通過形態(tài)觀察、生理生化試驗及16S rDNA 同源性序列分析，鑒定該菌為解淀粉芽孢桿菌。通過對By33 代謝產(chǎn)物對TMV 抑制作用測定發(fā)現(xiàn)，其代謝物質(zhì)對溫度敏感，80℃處理30 min，喪失大部分活性，遇酸沉淀，堿性條件下穩(wěn)定，對蛋白酶不敏感。

       

      Abstract: An antagonistic bacteria strain By33 against TMV was isolated from tobacco soils. Basing on morphological observation, physiological & biochemical test and 16S rDNA sequence analysis, By33 was identified as Bacillus amyloliquefaciens. The active substance lost most active control at 80 ℃, and was unstable in acid condition yet stable in neutral or slight alkaline condition, with resistant to proteolytic enzymes.

       

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