拮抗細(xì)菌AR03 在煙草根部及根際土壤中的定殖與鑒定

Identification and Colonization of an Anti-bacteria AR03 in Root and Rhizosphere Soils

摘要: 以抗利福平為標(biāo)記，采用澆灌根基部接種的方法測(cè)定AR03 菌株在煙草植株根際的定殖。結(jié)果表明，在滅菌土條件下，AR03 經(jīng)人工引入可以有效的定殖于煙草根際土壤和根部，并在一定時(shí)間內(nèi)可維持相當(dāng)?shù)臄?shù)量，在根際土壤和根部的定殖動(dòng)態(tài)趨勢(shì)是先升后降；它們?cè)诟康亩ㄖ沉雀H土壤中強(qiáng)。依據(jù)傳統(tǒng)形態(tài)學(xué)、生理生化特征及16s rDNA 基因序列，菌株AR03 為短小芽孢桿菌(Bacillus pumilus)。

Abstract: Tagged by the resistance to Rif.(300 μg/mL) and inoculated by watering root, under the sterilized soil condition, the rhizosphere bacteria AR03 strain was proved that it could colonized in root and rhizosphere soils of tobacco by artificial induction. AR03 strain could maintain considerable quantity for a while. The colonized dynamics of AR03 strain increased first, then decreased. Besides these, the colonization was greater on root than in rhizosphere. On the basis of observation of colony and cell morphology, biochemical test and the 16s rDNA sequence analysis, Strain AR03 was Bacillus pumilus.